307-34-6

Appearance：colourless liquid

Density：1.766

Boiling Point：103-104ºC

Melting Point：-25ºC

Flash Point：30.7ºC

Refractive Index：1.3

Stability：Stable.

Storage Condition：Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances.

CAS NO:307-34-6

Methylene Blue is a synthetic aromatic chemical compound of phenothiazine type. It is an azo compound with the structure of two pyridine-like heterocyclic rings linked by a 3-carbon chain. It is an intensely blue dye used as a stain for microscopic observation, and as a histological marker for amoebic cysts. It is also used to test for herpetic lesiones on the eyes, to differentiate red blood cells in a high-power field, to measure pH values in semi-micro, and to test water and urine samples at normal dilutions. 

Methylene Blue is an amphoteric substance and can be reduced to leuco-methylene blue by reduction agents such as hydroxylamine, hydrazine, hydrazine sulfate, and aromatic amines. It is also used in analytical chemistry and to measure the hematocrit of blood samples. It can also be used as an indicator of cytoplasmic alkalinity, an aqueous methemoglobin test and in qualitative analysis to distinguish substances with different colors.

Methylene Blue is used extensively in the dye industry, hair coloring agents, antiseptics, and in redox reactions. It can be used to dye or tint fabrics, paper, wool, cotton and some synthetics. It can also be used as a discoloring agent for indigo and alizarin dyes. In the medical industry, it is used as a topical antibacterial agent, as a hemostatic agent, and in disinfectants, biocides and vaccines.

Methylene Blue is highly poisonous and corrosive, with an LD50 (lethal dose for 50% of the test population) of 900mg/kg (orally in rats). It should be kept in a well-ventilated area, away from any source of heat and away from direct sunlight. It should only be handled in a laboratory by people wearing protective clothing and goggles.